Investigation of Antioxidant Activities of Pleurotus ostreatus Grown in Different Composts

Abstract

In many countries of the world, the use of organic waste such as tea leaves and wood chips in the cultivation of P. ostreatus prevents both these materials from being wasted and contributes to the provided income. In this study, the availability of infused tea leaf in the production of Pleurotus ostreatus was investigated. Six different experimental groups and I control group were prepared by using "cotton", "siyez", "bran", "infused tea leaf', "poplar sawdust": 1st Group (5o% cotton + 5% siyez + 5% bran + 3o %infused tea leaves + io% poplar sawdust), 2nd Group (5o% cotton + 5% siyez + 5% bran + 4o%infused tea leaves), 3rd Group (5o% cotton + 5% siyez + 5% bran + io% infused tea leaves + 3o%poplar sawdust), 4th Group (50% cotton + 5% siyez + 5% bran + 20% infused tea leaves + 20% poplar sawdust), 5th Group (i00% infused tea leaves), 6th Group (9o% infused tea leaves + 5% siyez + 5% bran) and Control (7th) Group (5o% cotton + 5% siyez + 5% bran + 4o% poplar sawdust). In this study, DPPH (1, 1 diphenyl-2-picryl hydrazyl) radical scavenging method, which is widely used for the determination of antioxidant activity of Pleurotus ostreatus grown in different compost media, was used. Antioxidant activity of mushroom extracts were expressed as percentage of DPPH radicals inhibition and IC, values (mg/ml). Percentage of inhibition ranged from 16.54 to 91.34 % and IC, values ranged from 0.592 to 1.133 mg/mL for mushroom samples in different composts. The total phenolic content ranged from 84.79 to 169.58 mg/ g for mushroom extracts. The content of phenols in methanolic extracts expressed in gallic acid equivalents (GAE) varied between 84.79 ±1.15 and 169.58 ±2.10 mg/g. It was also observed that the antioxidant activity of the specimens grown especially in tea compost (l00%) was highest. According to the IC„ values, the order of antioxidant activity was determined as 5th Group> 3rd group> 2nd Group> 6th Group> 1st Group> 4th Group> 7th Group from large to small.